Table 2.
Kinetic-Langmuir approximations of the affinity of S9.6 scFv binding to hybrids obtained via SPR.
| Nucleic Acid Hybrid | ka (× 106 1/M·s)a | kd (× 10−3 1/s) | KD (× 10−9 M, nM) |
|---|---|---|---|
| 23AURR | 0.44 ± 0.03 | 1.19 ± 0.07 | 2.7 ± 0.4 |
| 23ATDR | 2.2 ± 0.3 | 1.2 ± 0.4 | 0.6 ± 0.1 |
| 23GCDR | 1.3 ± 0.3 | 1.0 ± 0.1 | 0.8 ± 0.2 |
| 23MDR | 3.22 ± 0.06 | 1.5 ± 0.3 | 0.47 ± 0.08 |
| 15MDR | 2.6 ± 0.3 | 1.4 ± 0.2 | 0.54 ± 0.02 |
| 10MDR | 3.2 ± 0.1 | 1.6 ± 0.3 | 0.50 ± 0.06 |
| 8MDR | 3.1 ± 0.4 | 1.6 ± 0.2 | 0.51 ± 0.01 |
| 6MDR | 1.00 ± 0.06 | 1.81 ± 0.04 | 1.82 ± 0.09 |
a
Averages and standard deviations for all parameters are derived from 3 trials.