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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Apoptosis. 2014 Mar;19(3):467–479. doi: 10.1007/s10495-013-0944-1

Fig. 1. CPT induces MKP-1, JNK1/2 and ERK1/2 during apoptosis.

Fig. 1

(a) Confluent serum starved cells grown as described in the methods were left untreated (UT) or exposed to CPT (20 μM) for the indicated time intervals. Whole cell extracts (25μg) were subjected to 10% SDS-PAGE followed by western blot analysis to detect MKP-1, and phosphorylated JNK1/2 (p-JNK1/2) and ERK1/2 (p-ERK1/2) using specific antibodies. Actin immunoblotting was performed as an internal control for equal loading. Blots shown are representative of 3 observations. (b) Confluent serum starved cells treated as described above were used to determine apoptosis. DNA fragmentation was measured using a colorimetric ELISA kit as described in methods. Values are means ± SE of triplicates. *, p < 0.05 compared with UT.