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. 2013 Sep 16;3(3):1374–1394. doi: 10.3390/brainsci3031374

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© 2013 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Longitudinal/transverse/oblique sections of uninjured optic nerve fibers fixed and processed using the pyroantimonate procedure to localize free calcium. Scattered along the length of the myelin sheaths of nerve fibers are foci of oval profiles at which myelin lamellae are separated such that individual lamellae are visible. These represent myelin discontinuities (md) (white arrows). Within several mds electron dense pyroantimonate precipitate occurs (black arrows) indicating the occurrence of free calcium therein. Mitochondria (mt) with a characteristic cristate ultrastructure and longitudinally orientate microtubules (double arrows) occur within the axoplasm.