Table 5.
Description of the characterized β-1,4-xylanases isolated from the culture broth of a M. thermophila C1 mutant strain.
| Enzyme | CAZy module | MW-monomer (kDa) | pHopt | Topt (°C) | pI | Gene | References |
|---|---|---|---|---|---|---|---|
| Xyn10A | GH10 | 42/31* | 5.5–7.0 | 65–70 | 7.8/8.9 | xyl1 | Ustinov et al., 2008; van Gool et al., 2012 |
| Xyn10B | GH10 | 57/46* | 5.5–7.0 | 80–85 | 4.4/4.3 | xyl3 | Ustinov et al., 2008; van Gool et al., 2012 |
| Xyn10C | GH11 | 40 | 5.0 | 80 | 4.8 | xyl4 | Ustinov et al., 2008 |
| Xyn11A | GH11 | 24 | 6.5 | 70 | 7.9 | xyl2 | Ustinov et al., 2008 |
| Xyn11B | GH11 | 23 | 6.0–6.5 | 65–70 | 8.4 | xyl6 | Ustinov et al., 2008 |
| Xyn11C | GH11 | 22 | 4.5 | 65 | 6.7 | xyl5 | Ustinov et al., 2008 |
| Xyl7 | GH11 | 22/30* | 5.5–6.5 | 50–60 | 7.3/7.6 | xyl7 | van Gool et al., 2013 |
| Xyl8 | GH11 | 22 | 5.5–6.0 | 50–65 | 6.2 | xyl8 | van Gool et al., 2013 |
They display optimal activity at temperatures between 50 and 70 °C, increasing fungi's hydrolytic efficiency in various temperatures. Marked proteins
(*)
were isolated in two different forms, with (high molecular weight enzyme) or without CBM (low molecular weight enzyme).