Skip to main content
. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Methods Mol Biol. 2014;1126:169–177. doi: 10.1007/978-1-62703-980-2_13

Figure 1.

Figure 1

Coupled RNAP II transcription and pre-mRNA splicing in vitro. (A) Structure of the DoF CMV-DNA template. The sizes of the exons and intron are indicated. B. 32P-UTP and the CMV-DoF DNA template were incubated under transcription/splicing conditions for 8 minutes. α-amanitin was added after the 8-minute time point and incubation was continued for the indicated times. Pre-mRNA and the splicing intermediates are indicated.The endogenous U6 snRNA and tRNA in the extract are labeled by the 32P-UTP (see Reddy et al. [1987].