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. 2014 May 18;7:304. doi: 10.1186/1756-0500-7-304

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Copyright © 2014 Ray and Johnson; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Validation of candidate reference genes in Barnea, Frantoio and Picual independently using GeNorm algorithm in qBase Plus. (A) Average expression stability values (M) of the eight reference genes plotted from least stable (left) to most stable (right) in Barnea (A), Frantoio (C) and Picual (E) (B) Pairwise variation analysis (V_n/V_n+1) between the normalisation factors NFn and NFn + 1 to determine the optimal number of reference genes to be used for normalisation against target genesin Barnea (B), Frantoio (D) and Picual (F).