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Amino acid sequence alignment of the corrected RcKS1, RcKSL2, RcKSL3 and corrected RcKSL4 (corrections as described in the text). The primary DDxxD and secondary (N/D)Dxx(S/T)xxxE divalent magnesium ion binding motifs are underlined, with an asterisk (*) underneath the previously identified single residue switch position controlling progression to the secondary cyclization step of the KS reaction mechanism.
