Figure 6.

Standard histology of conventional outflow tissues in mouse eyes treated with pilocarpine. Schlemm's canal lumen of mouse eyes treated with pilocarpine (PILO) or PBS (CON) were examined. Eyes were treated and then fixed by immersion or perfusion at one of three intraocular pressures (10, 25, or 45 mm Hg). Data show that SC lumen is more dilated regardless of fixation technique or perfusion pressure compared with control. Data are representative of images captured from two quadrants of two eyes for each condition. Arrows indicate pectinate ligaments with attached lamellated TM. The widening of the TM and anterior chamber angle was not evaluated in these histological sections because of presumably artificial changes in the position of the iris and ciliary body during processing on account of the large lens.