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. 2014 Jun 17;55(6):3775–3785. doi: 10.1167/iovs.14-13889

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Copyright 2014 The Association for Research in Vision and Ophthalmology, Inc.

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An incomplete BBSome complex is assembled in the CCT-deficient rods. Whole-eye extracts from ^Δ1–83PhLP-FLAG^−/− and ^Δ1–83PhLP-FLAG^± littermates at P10 were fractionated by ultracentrifugation on a 10% to 40% sucrose gradient calibrated using sedimentation (S) coefficient standards. Collected fractions were analyzed by Western blotting using specific antibodies against the indicated BBS proteins. A mature BBSome contains BBS1, -2, -4, -5, -7, -8, and -9 and migrates as a 14S complex (▴); an incomplete BBSome contains BBS1, -4, -8, -9, and migrates as a 9S complex (Δ).