Skip to main content
. 2014 Jun 18;9(6):e99593. doi: 10.1371/journal.pone.0099593

Figure 5. Morphological and immunophenotypical analyses of primary cultures from 15-month regenerated allogeneic walls.

Figure 5

I. Two main morphologies could be observed during culturing medial cells: a cell line more elongated reaching confluence with hill and valley distribution (A, B and C) and another one characterized by spindle-shaped cells growing in clusters (E, F and G). Such morphological aspects revealed a fine analogy of these two primary cells respectively to mature artery SMCs (D) and bone marrow mesenchymal stem cells (H), both of porcine origin. Magnifications: (A, E) 150 µm, (B, F) 100 µm, (C, D, G, H) 50 µm. II. Immunophenotype of primary cultures from 15-month regenerated allogeneic walls. Wide FITC-conjugated SMA-positivity (green), less usual co-immunodetection with smoothelin or SM-MyHC (in red) (A–E), rare OC positivity (F), extensive CD29 expression (J) in contrast to SSEA4, OCT4 and CD90 (G–I) (in green). Magnification: 30 µm.