Table 1. Immunophenotype of primary cultures derived from explanted valve allografts.
| Antigens | Medial cells (%) | Adventitial cells (%) | VICs (%) |
| Pig CD45 | ND | 0,00±0,01 | 0,00±0,01 |
| MyHC-Apla1 | 82,51±5,30 | 87,06±8,21 | 95,26±1,93 |
| Calponin | 26,57±3,69 | 11,30±7,88 | 7,11±0,97 |
| SM-MyHC | 28,15±9,01 | 29,71±13,61 | 5,02±2,96 |
| Smoothelin | 31,03±7,16 | 12,56±6,15 | 3,41±2,00 |
| OPN | 68,06±16,33 | 63,41±13,28 | 88,91±7,02 |
| OC | 1,25±0,18 | 1,06±0,05 | 0,98±0,23 |
| SSEA4 | 15,89±4,02 | 7,41±5,38 | 20,26±4,59 |
| OCT4 | 7,06±6,99 | 10,06±4,45 | 29,97±1,81 |
| CD90 | 3,10±2,01 | 1,15±0,86 | 25,80±2,22 |
| CD29 | 74,36±4,13 | 52,29±22,78 | 88,30±11,01 |
Legend: Percentage of antigen expression after normalization to counted nuclei. ND: not detectable.
Primary cultures extracted from explanted tissues at 15 months were characterized immunophenotypically for markers commonly expressed in inflammation (CD45), SMCs and/or VICs (OPN, MyHC-Apla1, Calponin, SM-MyHC and smoothelin), calcification (OC) and stemness (SSEA4, OCT4, CD90 and CD29).