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. 2014 Jun 18;9(6):e99884. doi: 10.1371/journal.pone.0099884

Figure 5. Stage specific expression of wBm-MurA gene and the enzyme.

Figure 5

A: Expression of wBm-MurA gene. The full-length DNA (1278 bp, wBm-MurA gene) was amplified from the cDNA of three life- stages of B. malayi using gene specific primers. Lane 1, molecular size markers (GeneRuler 1 kb DNA Ladder, Thermo Scientific); lane 2, infective larvae; lane 3, adults (both sexes); lane 4, microfilariae. Lane 5, 6 and 7 are controls containing PCR products from infective larvae, adults and microfilariae respectively in absence of reverse transcriptase. B: Endogenous protein (wBm-MurA) expression. Western blot was performed with anti-wBm-MurA antibody to confirm the presence of wBm-MurA. Lane 1, molecular mass markers (Puregene 4 Color Prestain protein ladder, Genetix); lane 2, microfilariae; lane 3, infective larvae; lane 4, adult worms (both sexes); and lane 5, purified wBm-MurA protein (positive control).