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. 2014 Jun 18;9(6):e99946. doi: 10.1371/journal.pone.0099946

Figure 2. CM from osteoblasts lacking Vhl increases BMSC-derived osteoblast differentiation,and decreases BMSC-derived adipogenesis.

Figure 2

(A) Measurement of osterix, Runx2, ALP and OC mRNA expression by quantitative real-time PCR at day 14 of osteogenic induction. (B) Alizarin Red S (Sigma-Aldrich, St. Louis, MO) staining of BMSCs cultured with CMs at day 21 of osteogenic induction. (C) Numbering of mineralized nodules of B. (D) The mRNA level of PPAR-γ and C/EBP-α of BMSCs at day 14 of adipogenic differentiation. Data represent mean ± SD. *,p<0.05; **,p<0.01, ***,p<0.001.