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. 2014 Jun 18;9(6):e100372. doi: 10.1371/journal.pone.0100372

Figure 4. AEG-1 regulates downstream substrates of Akt in HCC cells under suspension conditions.

Figure 4

The AEG-1-over-expressing and AEG-1-knockdown cells were grown under suspension conditions. Cell lysates were prepared 24 h after suspension culture and immunoblotted with the indicated antibodies. GAPDH is shown as a loading control. Representative results are shown (left), and the mean +SD of the signal intensity of protein bands in three independent experiments is quantified (right; *P<0.05 vs corresponding vector or siRNA control).