Figure 3. Whole kidney gene expression (mRNA) comparisons as measured by quantitative RT/PCR (qRT-PCR) for wild type (WT) and HYP mice infused with vehicle or SPR4 peptide for 28 days.

Column headings represent; WT  =  wild type mice, HYP  =  X-linked hypophosphatemic rickets mice, SPR4  =  infused SPR4-peptide and Vehicle  =  Saline infused. For gene analysis mRNA was prepared from whole kidneys snap frozen in LN2 and homogenized. For qRT-PCR gene analysis fold differences in expression calculated by the Pfaffl method [163] were statistically analyzed for significance using the One Sample t-test and the Wilcoxon Signed rank-test with theoretical means set to 1. Results are significant (*  =  p<0.05) unless indicated by NS (see also Table 3 for detailed statistics). ND =  Not done, NS  =  Not SignificantIndex: Cyclophilin  =  cyclophilin; GAPDH  =  Glyceraldehyde 3-phosphate dehydrogenase; SOST  =  Sclerostin; VEGF  =  Vascular Endothelial Growth factor; 24-Hydroxylase  =  1,25-hydroxyvitamin D₃ 24-hydroxylase (CYP24A1); 1-á-Hydroxylase  =  25-hydroxyvitamin D₃ 1-alpha-hydroxylase (CYP27B1); NPT2c  =  Sodium-dependent phosphate co-transporter (Slc34a3); NPT2a  =  Sodium-dependent phosphate co-transporter (Slc34a1); NS  =  not significant; *  =  P<0.05. Histogram bars to the left of zero on the axis indicate down regulation and to the right up regulation.