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. 2014 Jun 18;9(6):e100343. doi: 10.1371/journal.pone.0100343

Figure 2. Mapping of nucleosome positions at the promoter and upstream region of OsDREB1b locus.

Figure 2

(A). Chromatin isolated from 2–3 weeks old rice seedlings were partially digested with increasing concentration of MNase, and probed with DNA from the core promoter region (−74 to −232) (B) Determination of the nucleosome positions at the promoter and upstream region OsDREB1b locus by PCR based approach. The positions of the nucleosomes are given relative to the OsDREB1b locus. G denotes genomic DNA, M denotes mono-nucleosomal DNA and D denotes di-nucleosomal DNA. Black shaded ovals denote a well positioned nucleosome and a grey shaded oval represents a partially positioned nucleosome.