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. 2014 Jun 18;9(6):e100343. doi: 10.1371/journal.pone.0100343

Figure 6. Change in DNase I accessibility at the promoter and upstream region of OsDREB1b loci.

Figure 6

Relative DNase I accessibility in control and cold stress treated nuclei (2 Hr and 4 Hr) was detected with PCR based method. Nuclei were digested with DNase I (5 U/ml) for increasing time period (0,3,6,10 min). The isolated DNA was used for PCR reaction with primers specific for promoter and upstream region. The amount of DNA amplified at each time point was normalised to that at time 0 and plotted against time to compare the rate of degradation. The relative rate of accessibility for actin promoter (A and D) and OsDREB1b (B, C, D and E) and The data represented here is a mean of three independent experiments with standard error bars. Statistically significant values were marked with *.