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(A–E) Double immunostaining of 4-HNE with cellular markers for neurons (A), astrocyte (B), oligodendrocyte (C, D), and microglia (E). 4-HNE signals were mainly colocalized with the oligodendrocyte marker NG2 and olig2, but not with NeuN, GFAP, and Iba1 in the spinal cord at the end of 4 days period after PSNL. Scale bar: 20 µm.
