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. 2014 May 23;30(23):6897–6905. doi: 10.1021/la500766t

Figure 3.

Figure 3

(A) Exemplary QCM curves of the fifth overtone showing the binding of streptavidin to biotinylated PEG monolayers. QCM crystals are PEGylated with different mol % of clickable PEG-alkyne and clicked with biotin-azide prior to the measurements. The QCM crystals are equilibrated with PBS for 30 min, incubated with 5 μg/mL streptavidin in PBS for 1 h, and subsequently rinsed with PBS for 30 min to remove unbound streptavidin. (B) Frequency change for surfaces of different compositions after streptavidin binding. The errors are the standard deviation from at least three independent experiments.