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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Nat Chem Biol. 2014 May 18;10(7):552–554. doi: 10.1038/nchembio.1527

Figure 2.

Figure 2

Genetic confirmation of CcsB activity. A) i) and iii): HPLC analysis (λ= 210 nm) of metabolites extracted from OE::ccsR Δlig4 strain and from ΔccsB-37 mutant, respectively; ii) and iv): Selected ion monitoring of m/z: 518 (1, 2), 486 (5), and 454 (7) of traces i) and iii), respectively. All m/z values are [M+Na]+. The metabolites are extracted from the 4th day cultures. The experiments were performed three times and a representative result is shown here. B) Chemical structure of 7.