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. Author manuscript; available in PMC: 2015 Jul 18.
Published in final edited form as: Chem Commun (Camb). 2014 Jul 18;50(56):7424–7426. doi: 10.1039/c4cc02000f

Figure 2.

Figure 2

(A) The site-specific incorporation of 1 into sfGFP at its S2 position. Cells transformed with pEVOL-PylRS(N346A/C348A) and pET-pylT-sfGFP2TAG were grown in conditions with or without a NCAA supplement. (B) The deconvoluted ESI-MS spectrum of the purified 1-containing sfGFP. Its theoretical value is 27,756 Da.