Results of registration for TEM/confocal images using MI similarity measure with directly registration (first row) and our proposed IA method (second and third rows) using (a, d, g) rigid registration (b, e, h) affine registration and (c, f, i) b-spline registration. The results are shown in a checkerboard image for comparison. Here, first and second rows show the checkerboard images of the original TEM/confocal images 1 while the third row shows the checkerboard image of the results of our proposed IA method. Differences are generally small, but some improvements can be observed for B-spline registration.
1The grayscale values of original TEM image are inverted for better visualization