Fig. 5.

Cytotoxicity effect of hydroxyurea and hydroxyurea loaded into nano conjugate complex on MCF-7 cell line after 48 h of incubation. Results are presented as mean ± 5 % error of three independent experiments. Reducing cytotoxicity by decreasing drug concentration was also observed for nano conjugates which was valid for concentrations higher than 40 μM. Concentrations lower than 40 μM (i.e. gold NPs concentration was lower than 2 μM) demonstrated enhancing cytotoxicity in spite of reducing drug concentration