Fig. 2.

Inward rectification of L906C is independent of divalent cations. Similar to Fig. 1b, c, but the recording was performed using a Ca²⁺-free (omitting Ca²⁺ and adding 0.5 mM EGTA) bath solution (a, b) or divalent cation-free bath (omitting Ca²⁺ and Mg²⁺ and adding 0.5 mM EGTA) and pipette (omitting Ca²⁺ and Mg²⁺ and adding 10 mM BAPTA) solutions (c, d). Representative traces are shown as for Fig. 1b, c for cells that expressed wild-type mouse TRPA1 (a, c) or its L906C mutant (b, d)