Figure 5.

Effect of Nef on lipid accumulation in aorta of C57BL/6 mice. A, B) Representative micrographs of sections of aortic sinus from mice treated with vehicle (A) or Nef (B) and stained with Oil Red O. C, D) Representative micrographs of sections of thoracic aorta from mice treated with vehicle (C) or Nef (D) and stained with Oil Red O. E) Representative micrograph, high magnification, of a section of aorta of Nef-treated mice after staining with Oil Red O. F) Representative micrograph, high magnification, of a section of aorta with low abundance of lipid staining of Nef-treated mice after staining with Oil Red O. Inset: detail of G) Representative micrograph, high magnification, of a section of aorta with low abundance of lipid staining of Nef-treated mice after staining with anti-CD68. Inset: detail of cell indicated by arrow in panel. Scale bars = 50 μm. H) Quantitation of the area occupied by lipid inclusions in aortic arch region after staining of sections with Oil Red O. *P < 0.01 vs. vehicle. I) Quantitation of the area occupied by lipid inclusions within the thoracic aorta after staining of sections with Oil Red O. *P < 0.001 vs. vehicle. J) Quantitation of the area occupied by lipid inclusions within the whole aorta after staining of sections with Oil Red O. *P < 0.01 vs. vehicle. K) Comparison of the area occupied by lipid inclusions within the whole aorta after 12 and 20 wk after staining of sections with Oil Red O. Circles, control; squares, Nef. *P < 0.05, ***P < 0.01.