Figure 7.

ROCK is a negative regulator of ULF transport along microtubules. A) F-actin staining of GFP-ULF cells fixed 1 h after treatment with 10 μM Y-27632. Note that the Y-27632 treatment inhibited actin bundling into stress fibers but did not affect ULF interaction with F-actin. B) Lat B-treated GFP-ULF cells were treated with 10 μM Y-27632 for 1 h before and during live-cell imaging of GFP-ULF motility. Temporal color coding of ULF position revealed the presence of very long trajectories in Y-27632-treated cells as compared to control. Scale bars = 10 μm. C) Graph of the frequency distribution of ULF trajectory length shows that Y-27632 treatment increased the frequency of motile ULFs and the length of trajectories.