Figure 7.

Role of paxillin phosphorylation at Tyr³¹ an Tyr¹¹⁸ in CS-induced EC permeability. Human pulmonary ECs were transfected with wild-type (Pxn-FL) or phosphorylation-deficient mutant (Pxn-Y31/118F). A) GEF-H1 activation was evaluated by GEF pulldown assay. Western blot detection of GEF-H1 in corresponding total lysates was used as normalization control. B) Phosphorylation of MYPT, MLC, and p44/42 MAPK was evaluated by Western blotting with corresponding phospho-antibody. Probing for β-actin was used as normalization control. Bar graphs depict the quantitative analysis of Western blot densitometry data. *P < 0.05 vs. paxillin wild type; n = 3. C) Permeability measurements of EC monolayers exposed to 18% CS, 15 min, were performed using FITC-avidin fluorescence XPerT assay. *P < 0.05 vs. paxillin wild type; n = 5.