Fig. 3.

Involvement of the Ca²⁺-activated K⁺ channel (Gardos channel) in Ca²⁺-induced PS exposure in RBCs from SCD patients. RBCs were treated with ionophore for 30 min (as in Fig. 1b). a RBCs were incubated in either HK or LK HEPES-buffered saline (HBS) in the absence and presence of 20 μM clotrimazole (CLT). Symbols represent means ± SEM, n = 5; black circles LK HBS, black squares LK HBS with CLT (20 μM), grey triangles HK HBS and grey diamonds HK HBS with CLT (20 μM). *p < 0.03 between PS exposure in RBC incubated in LK saline in the presence and absence of CLT, ^# p < 0.05 between LK and HK saline. b RBCs were incubated in HK or LK saline, in the absence and presence of charybdotoxin (600 nM). Histograms represent means ± SEM, n = 3. *p < 0.05