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Karyotype analysis. SD-Tg.EC1, p16 metaphase chromosomes were stained with Giemsa-trypsin. a Cells cultured in media containing source A CHIR99021 have a normal karyotype (42, XY). b Cells cultured in media containing source B CHIR99021 have an abnormal karyotype (41, XY, −14). n = 20 metaphase spreads analyzed per culture.
