Table 2.

Significant chemical species observed in LCMS data for CHIR99021 from source B

RT (min)	Species	Ions observed (m/z)	No. of Cl atoms	Diagnostic fragments (m/z)	Peak area at 340 nm, ×103
23.60	A	296/298	2	173/175	2.6
24.95	B	321	0	146	21.6
25.42	C	320/322	2	–	1.4
27.75	D	321/323	2	175, 147, 120	36.7
32.95	E	465/467	2	146	1742
34.35	F	479/481	2	146	114.8
35.90	G	466/468	2	146	23.7
38.80	H1	429/431	1	146	27.1
39.15	H2	429/431	1	146	17.7