Fig. 11.

Downregulation of TMEM16A suppresses intracellular Ca²⁺ ([Ca²⁺]_i) rises in response to NA and K⁺, partly as a consequence of reduced expression of vascular L-type calcium channel (Ca_V1.2). a Changes in [Ca²⁺]_i in response to increasing concentration of NA. [Ca²⁺]_i signaling is normalized to averaged maximal [Ca²⁺]_i level obtained in arteries transfected with eGFP-siRNA after 10 μM NA stimulation (n = 6). *P < 0.05, **P < 0.01, ***P < 0.001. b Ca²⁺ sensitivity expressed as a relation between wall tension and normalized [Ca²⁺]_i, as shown in a (n = 6). c Changes in [Ca²⁺]_i, in response to 60 mM K⁺-induced depolarization and 10 mM caffeine stimulation. [Ca²⁺]_i signaling is normalized to averaged maximal [Ca²⁺]_i level in arteries transfected with eGFP-siRNA after 10 μM NA stimulation (n = 6). *P < 0.05. d Expression of Ca_V1.2 in TMEM16A-downregulated arteries and eGFP-siRNA-transfected arteries (n = 6). **P < 0.01