Fig. 9.

Downregulation of TMEM16A does not affect the expression of α1-adrenergic receptors. a Relative mRNA level of α1a, α1b, and α1d isoforms of adrenergic receptors in arteries transfected with either Tmem16a-siRNA or siRNA directed against eGFP (eGFP-siRNA). Tmem16a expression was set to 100 % in nontransfected arteries isolated from the same rat. n = 4. No significant difference between the groups was found. b The average intensity of BODIPY-prazosin staining (representative images in c) in the SMCs of the outer layer of the media (SMC-OL) and the inner layer (SMC-IL) normalized to the intensity observed in the nontransfected artery (set to 100 %). No significant difference between nontransfected arteries and arteries transfected with either Tmem16a-siRNA or eGFP-siRNA was found. c Representative BODIPY-prazosin staining of Tmem16a-siRNA-transfected arteries (i), eGFP-siRNA-transfected arteries (ii), and nontransfected arteries (iii) for BODIPY-prazosin staining and corresponding differential interference contrast image for the same artery. Scale bar represents 50 μm in all images