. 2013 Oct 27;466(7):1391–1409. doi: 10.1007/s00424-013-1382-1

Table 1.

Oligonucleotide sequences

Primer (sequencing experiments)	Sequence	Target
rTM_47-66 F	catcatgcaggacacacagg	Bp 47–^a
rTM_751-770 F	tgcccacgaagaaggtgtac	Bp 751–^a
rTM_1555-1574 F	ccttcatggagcactggaaa	Bp 1555–^a
rTM_1861-1880 F	tgaactcctccccatctgtg	Bp 1861–^a
rTM_2540-2559 F	agccatcagagccaaagaca	Bp 2540–^a
rTM_3361-3380 F	aggccattatgcagggaagt	Bp 3361–^a
rTM_747-766R	accttcttcgtgggcatctt	Bp −766^a
rTM_1054-1073R	cgccgtttacttagaagggc	Bp −1073^a
rTM_1861-1880R	cacagatggggaggagttca	Bp −1880^a
rTM_3090-3109R	ctgtgggactgtggttgtta	Bp −3109^a
rTM_3694-3713R	ccaggcttatttctgaccct	Bp −3713^a
rTM_4600-4619R	gaacgctcagccacagactc	Bp −4619^a
siRNA	Sequence
Tmem16a-siRNA (exon 23)	cggucuucuuucuucuuucTT	Ano1/NM_001107564.1^b
mutated Tmem16a-siRNA	cggucggcuuacaucuuucTT	Ano1/NM_001107564.1^b
Tmem16a-siRNA-2 (exon 22)	guuuuuaucuuuaguauucTT	Ano1/NM_001107564.1^b
eGFP-siRNA	ccacuaccugagcacccagTT	eGFP^b
Primer (qPCR)	Sequence
rTM_taq2621-F	tgtgatctccttcacgtctg	Ano1/NM_001107564.1^b
rTM_taq2825-R	catacttgtgttctgaccacg	Ano1/NM_001107564.1^b
rCacna1c-F	catctccatcaccttcttccg	Cacna1c/NM_012517.2^b
rCacna1c-R	aaatacctgcatcccaatcactg	Cacna1c/NM_012517.2^b
α1a α-adrenergic receptors-F	ccgttcttcctagtcatgcc	Adra1a/NM_0171981.2^b
α1a α-adrenergic receptors-R	atgtctctgtgctgtccctc	Adra1a/NM_017191.2^b
α1b α-adrenergic receptors-F	caagaactttcatgaggacaccc	Adra1b/NM_016991.2^b
α1b α-adrenergic receptors-R	ccagaacaccaccttgaacac	Adra1b/NM_016991.2^b
α1d α-adrenergic receptors-F	cggaccacagagtagcaagg	Adra1d/NM_024483.1^b
α1d α-adrenergic receptors-R	tagcccagccagaagatgac	Adra1d/NM_024483.1^b
rBest1 (Applied Biosystems)	Rn01451107_m1	Best1/NM_001011940.1^b
rBest2 (Applied Biosystems)	Rn00520372_m1	Best2/NM_001108895.1^b
rBest3 (Applied Biosystems)	Rn01509681_m1	Best3/NM_001191783.1^b
rGAPDH_taq1000-F	cacggcaagttcaacggcacag	Gapdh/NM_017008.3^b
rGAPDH_taq1138-R	agactccacgacatactcagcacc	Gapdh/NM_017008.3^b
rTfrc_taq1708-F	agacagtaattggattagcaaaattg	Tfrc/NM_022712.1^b
rTfrc_taq1961-R	gggtcaactcaatgtcatgggta	Tfrc/NM_022712.1^b
Probe	Sequence
rTM_taq2690-P	acctctacatgtacagtcaaaacgggacc	Ano1/NM_001107564.1^b
Rcacna1c-P	tgctgtggaccttcatcaagtccttccagg	Cacna1c/NM_012517.2^b
rGAPDH_taq1040-P	agctggtcatcaacgggaaacccatcacca	Gapdh/NM_017008.3^b
rTfrc_taq1905-P	cagcagaggtggccggtcagttcattattaaa	Tfrc/NM_022712.1^b

^aTarget position

^bTarget gene