Table 2.
Functional characteristics of arteries in isometric experiments. In one group of experiments, we compared three different groups of arteries from the same rats: Tmem16a-siRNA-transfected arteries, arteries transfected with mutated siRNA, and nontransfected arteries. In other experiments, only eGFP-transfected arteries and nontransfected arteries from the same rats were compared
| Rats transfected with Tmem16a-siRNA and mutated siRNA (n = 7) | Rats transfected with eGFP-siRNA (n = 3) | ||||
|---|---|---|---|---|---|
| Nontransfected | Tmem16a-siRNA | Mutated siRNA | Nontransfected | eGFP-siRNA | |
| ID (μm) | 297 ± 5 | 305 ± 10 | 295 ± 11 | 303 ± 11 | 297 ± 7 |
| −logEC50 NA | 5.68 ± 0.07 | 5.27 ± 0.06***,### | 6.024 ± 0.04** | 5.87 ± 0.08 | 5.82 ± 0.14 |
| −logEC50 AVP | 4.99 ± 0.10 | 4.93 ± 0.09# | 5.20 ± 0.07* | 5.12 ± 0.05 | 5.07 ± 0.10 |
| −logEC50 NA + 20 mM K+ | 5.91 ± 0.06 | 5.72 ± 0.11*,### | 6.32 ± 0.08** | ||
| Max NA, N/m | 5.88 ± 0.40 | 2.03 ± 0.50***,## | 4.72 ± 0.48 | 4.81 ± 0.70 | 4.82 ± 0.97 |
| NA + 20 mM K+, N/m | 5.70 ± 0.40 | 2.20 ± 0.45***,# | 4.00 ± 0.46 | ||
| Max AVP, N/m | 5.16 ± 0.25 | 3.36 ± 0.44*,# | 5.73 ± 0.55 | 4.86 ± 0.61 | 4.99 ± 0.79 |
| Max K+, N/m | 5.40 ± 0.42 | 2.36 ± 0.44**,# | 4.20 ± 0.55 | ||
| Caffeine, N/m | 0.70 ± 0.10 | 0.60 ± 0.06 | 0.76 ± 0.11 | ||
| Relaxation to ACh, % | 68.01 ± 5.41 | 65.09 ± 6.17 | 63.18 ± 5.41 | 62.11 ± 5.12 | 65.75 ± 5.21 |
ID inner diameter, NA noradrenaline, AVP arginine vasopressin, ACh acetylcholine
*P < 0.05, **P < 0.01, ***P < 0.001 vs. nontransfected arteries; # P < 0.05, ## P < 0.01, ### P < 0.001 vs. arteries transfected with mutated, nonfunctional siRNA