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Substrates as short as 15-nt are cleaved by RISC. (A) Sequences of the truncated substrates. The 13-nt core sequence required for RISC-guided cleavage is shaded. (B) PhosphorImaging of cleavage reactions incubated for 1 h at 30°C and resolved on a 15% denaturing polyacrylamide gel. Substrate RNAs were radiolabeled only at the 5′ terminus. Arrows indicate the 7- and 9-nt 5′ cleavage product. The fraction of cleaved material is indicated at the bottom of the gel.
