Table 1.
Comparison of PBMC CD8+ TEM and gastric LPMC CD8+ TRM cells activation responses in adult, children, and the elderly.
| Cytokine | Stimulant | Adult |
Children |
Elderly |
|||
|---|---|---|---|---|---|---|---|
| CD8+ TEM | CD8+ TRM | CD8+ TEM | CD8+ TRM | CD8+ TEM | CD8+ TRM | ||
| Median | % (Range) | Median | % (Range) | Median | % (Range) | ||
| IL-2 | Media | 0.1 (0– 2) | 1.2 (0– 2)a,** | 0.3 (0–1) | 1.0 (0–2) | 0.1 (0– 1) | 1.7 (0– 3)** |
| SEB | 1.7 (0– 17) | 1.6 (0– 42) | 0.3 (0– 2) | 1.5 (1– 2) | 1.8 (1– 13) | 3.9 (1– 22) | |
| α-CD3/CD28 | 2.0 (0– 12) | 1.7 (0– 11) | 0.3 (0– 1) | 1.0 (0– 3) | 1.4 (1– 9) | 4.0 (1– 12) | |
| INF-γ | Media | 0.2 (0– 6) | 1.3 (0– 3)* | 0.4 (0– 3) | 1.6 (1– 3) | 0.6 (0– 2) | 1.7 (0– 5) |
| SEB | 8.6 (0– 37) | 15.7 (8– 27) | 4.3 (1– 19) | 10.3 (6– 17) | 6.3 (2– 57) | 11.5 (2– 28) | |
| α-CD3/CD28 | 3.1 (0– 19) | 13.8 (3– 30)** | 2.7 (1– 6) | 12.3 (6– 23)* | 6.5 (1– 15) | 20.9 (10– 28)* | |
| MIP-1β | Media | 0.2 (0– 6) | 1.9 (0– 8)** | 0.5 (0– 4) | 0.6 (0– 6) | 1.0 (0– 4) | 4.8 (1– 10)* |
| SEB | 5.0 (0– 20) | 15.6 (5– 31)* | 4.4 (0– 7) | 8.6 (3– 15) | 15.5 (1– 55) | 17.8 (3– 30) | |
| α-CD3/CD28 | 2.3 (0– 20) | 16.7 (3– 49)** | 1.4 (0– 14) | 8.9 (2– 42) | 14.6 (1– 39) | 33.2 (8– 55) | |
| TNF-α | Media | 0.2 (0– 3) | 1.4 (0– 4)* | 0.9 (0– 3) | 1.9 (1– 3) | 0.9 (0– 2) | 0.7 (0– 4) |
| SEB | 2.6 (0– 32) | 9.1 (4– 22) | 2.6 (2– 21) | 3.4 (1– 15) | 9.4 (1– 49) | 7.6 (1– 17) | |
| α-CD3/CD28 | 2.9 (0– 13) | 7.8 (2– 15)* | 1.9 (1– 5) | 8.5 (4– 11) | 9.9 (1– 21) | 9.7 (1– 13) | |
| IL-17A | Media | 0.1 (0– 2) | 0.7 (0– 2)* | 0.5 (0– 1) | 1.1 (0– 2) | 0.1 (0– 1) | 0.8 (0– 2) |
| SEB | 1.1 (0– 4) | 1.1 (0– 7) | 0.3 (0– 2) | 2.2 (0– 9) | 0.2 (0– 1) | 0.4 (0– 10) | |
| α-CD3/CD28 | 2.0 (0– 5) | 1.0 (0– 3) | 0.2 (0– 1) | 1.9 (0– 2)* | 0.2 (0– 1) | 0.9 (0– 5)** | |
| CD107a | Media | 0.1 (0– 4) | 1.4 (0– 3)** | 0.8 (0– 4) | 3.4 (2– 4) | 1.1 (0– 4) | 2.6 (1– 5) |
| SEB | 5.2 (0– 21) | 12.9 (7– 40)* | 4.6 (3– 21) | 9.8 (7– 23) | 14.2 (5– 45) | 12.9 (3– 26) | |
| α-CD3/CD28 | 1.3 (0– 12) | 10.8 (2– 18)** | 3.0 (2– 7) | 16.6 (7– 28)** | 12.7 (1– 21) | 12.3 (10– 19) | |
Gastric LPMC CD8+ TRM responses (IL-2, IFN-γ, MIP-1β, TNF-α, IL-17A, and CD107a) to two stimulants (SEB and α-CD3/CD28 beads) were compared to PBMC CD8+ TEM responses obtained from adults, children, and the elderly. Significant differences are shown in highlighted colors as determined by Mann–Whitney tests. Adults: n = 10; children: n = 10; elderly: n = 10.
a Light green color = significant increase in the frequency of CD8+ Trm (LPMC) compared to CD8+ Tem (PBMC); *p < 0.05; **p < 0.005.