Table 3.
Cytokine | Stim | PBMC | LPMC | PBMC | LPMC | ||||
---|---|---|---|---|---|---|---|---|---|
CD8+ TEM |
CDS+ TRM |
CD4+ TEM |
CD4+ TRM |
||||||
Cha | Elb | Ch | El | Ch | El | Ch | El | ||
IL-2 | Med | ||||||||
SEB | |||||||||
α-CD3/CD28c | * | ||||||||
IFN-γ | Med | * | |||||||
SEB | |||||||||
α-CD3/CD28 | * | ||||||||
MIP-1β | Med | *,d | |||||||
SEB | * | ||||||||
α-CD3/CD28 | * | ||||||||
TNF-α | Med | * | * | ||||||
SEB | ** | ||||||||
α-CD3/CD28 | * | ** | |||||||
IL-17A | Med | ||||||||
SEB | |||||||||
α-CD3/CD28 | |||||||||
CD107a | Med | * | |||||||
SEB | * | ||||||||
α-CD3/CD28 | ** |
LPMC CD8+ and CD4+ TRM and PBMC TEM responses (IL-2, IFN-γ, MIP-1β, TNF-α, IL-17A, and CD107a) to stimulation by two mitogens (SEB and anti-CD3/CD28) were determined and compared between children (Ch) or elderly (El) to adults (children vs. adult and elderly vs. adult). Significant differences are shown in highlighted colors as determined by Mann–Whitney test. Adults: n = 8; children: n = 5; elderly: n = 7. Empty (white) cells indicate non-significant differences between children or elderly vs. adults as determined by Mann–Whitney tests.
a Children; bElderly; cAnti-CD3/CD28 beads; dLight green color = significant in cytokine production compared to *p < 0.05; *p < 0.005.