Skip to main content
. 2004 May 1;18(9):992–1006. doi: 10.1101/gad.300004

Figure 1.

Figure 1.

Instability of a modified Chromosome VII-L telomere carrying 32-misoriented Rap1-binding sites. (A) Colony color assay using the ADE2 marker to detect VII-L telomere loss from strains disomic for Chromosome VII (left). The wild-type and modified copies of Chromosome VII-L present in test (top left) and control (bottom left) strains are shown. The modified copy of Chromosome VII-L carries either 32 misoriented (black arrows pointing right; test strain, top left) or correctly oriented (black arrows pointing left; control strain, bottom left) Rap1-binding sites, the URA3 and the ADE2 genes integrated at the ADH4 locus. White arrows represent native TG1-3 sequence. The strains are ADE3 and ade2Δ at their chromosomal loci. Examples of isolated colonies generated by either strain on YPD medium are shown on the right. (B) Schematic representation of the modified VII-L telomere (as in A) in strains disomic for Chromosome VII (top). The black arrowhead (pointing right) represents a synthetic Rap1-binding site in the “incorrect” orientation. (H) HindIII. (Bottom panel) The Southern blot analysis of telomeres containing the indicated number of Rap1 sites. Following HindIII digestion, the URA3 probe detects the endogenous ura3-52 locus, a diffuse telomeric band, and a faint ladder of bands in the case of 32 misoriented Rap1-binding sites.