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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: J Long Term Eff Med Implants. 2014;24(1):77–88. doi: 10.1615/jlongtermeffmedimplants.2014010118

Figure 6.

Figure 6

(a) Flow cytometry of Ficol separated human PBMCs labeled with CD14 and CD3 demonstrated 17% of primary human PBMCs were CD14+ monocytes. (b) After isolation using magnetic bead separation (AutoMacs, Milteni) the purity of CD14+ monocytes was increased to 95% and used for subsequent testing. (c) TNF-α and (d) IL-1β responses of isolated differentiated primary human monocyte/macrophages challenged with 5 μm sized particles after 1hr (TNF-α) and 24 hrs (IL-1β), (n=3 subjects). Maximal stimulation occurred at a dose of 5 particles/cell. Note p<0.05 where indicated by bars or by * indicating comparison to controls using one way ANOVA with Newman-Keuls Multiple comparison test of pairs.