Experimental Setup for Differential Conditioning Experiments
(A) Artificial “flower” targets. Each target consisted of a microcentrifuge tube and a ring of 5-mm-thick UV-visible transparent acrylic sheet (Perspex) on which were mounted inner and outer rings of linear polarizer. The targets for the “downward-facing with intensity contrast” (DFIC) treatment were identical to those in the “downward-facing” experiment, except that an additional layer of neutral density filter was attached to each ring.
(B) Setup in the experimental arena. An even distribution of different target types was maintained for each experiment, and individual targets were pseudorandomly shuffled between foraging bouts, between which the bee returned to the nest to deposit sucrose.
See also Figure S1.