Table 2.
Reciprocal regulation of endothelial cell gene expression by Akt and FKHR
| GenBank # | Gene name | Max fold change myrAkt/GFP | Max fold change FKHR/GFP |
|---|---|---|---|
| BT006809 | Fatty acid binding protein 4 (FABP4) | 0.1 | 7.09 |
| NM_001964 | Early growth response 1 (EGR1) | 0.12 | 7.12 |
| X96719 | C-type lectin superfamily member 2 (CLEC2) | 0.16 | 8.06 |
| NM_001147 | Angiopoietin 2 (Ang-2) | 0.18 | 17.01 |
| NM_001901 | Connective tissue growth factor (CTGF) | 0.21 | 3.12 |
| NM_007036 | Endothelial cell-specific molecule 1 (ESM-1) | 0.25 | 10.52 |
| NM_080760 | Dachshund homolog (Drosophila) | 0.25 | 3.58 |
| NM_021229 | Protein with strong similarity to netrin 4 | 0.27 | 3.71 |
| X07820 | Matrix metalloproteinase 10 (MMP 10) | 0.27 | 3.93 |
| NM_003775 | EDG8 | 0.32 | 3.48 |
| NM_002962 | Cytokine A2 | 13.07 | 0.11 |
| NM_005630 | Solute carrier family 21 | 8.65 | 0.26 |
| NM_030817 | Protein of unknown function | 7.46 | 0.28 |
| NM_006988 | ADAMTS1 | 7.44 | 0.44 |
| NM_139314 | Angiopoietin-like 4 | 6.16 | 0.13 |
| NM_198337 | Insulin induced gene 1 | 5.91 | 0.22 |
| NM_019058 | HIF-1 responsive RTP801 | 5.91 | 0.14 |
| NM_004609 | Transcription factor 15 | 5.06 | 0.09 |
HUVECs were infected with adenoviruses encoding either GFP or myrAkt for 16, 20, or 24 h. RNA was isolated and subjected to microarray analysis. Genes whose expression was either induced greater than fivefold or repressed greater than threefold by Akt and that we had previously shown to be regulated in the opposite direction by FKHR are listed. The number reported for the myrAkt/GFP ratio indicates the maximum fold change for that gene during the time course of the experiment (16, 20, or 24 h).