Figure 1.

Insulin interacts with a soluble receptor in plasma from control rats. (A) Western blots against insulin after native gel electrophoresis from an insulin standard (Ins) and a plasma sample from an adult male Wistar rat (C). Insulin staining for the plasma sample is not located at the same position as the insulin standard, displaying less migration in the gel for plasma insulin. (B) Insulin-receptor (alpha-IR) staining by Western blot after denaturing gel electrophoresis of previously insulin-immunoprecipitated plasma samples from two control rats (C1 and C2).