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Three-step purification of SIR from the supernatant of cultured hepatocytes. Chromatograms from Con-A affinity, gel filtration Superdex 200, and anionic exchange chromatographies. In each purification step, the faded fractions were pooled and assessed for α-IR by Western blot to follow purification. Finally, a representative Western blot of each collected fraction from the last purification step.
