Figure 1.
(a) DNA microarray targets were printed on NEXTERION® Slide A+ aminosilane coated slides. (b) DNA was UV-cross linked at 250 mJ and slides were dried for 20 min at 75 °C. (c) Microarrays were blocked with pre-hybridisation buffer (4× SSC, 1% SDS, and 10 mg·mL−1 BSA) for 45 min at 42 °C. (d) Specific fluorescent probes (Table 2) for DENV-1, -2, -3, or -4 (5 μL) were hybridised to the DNA microarray. (e) After washing, the slides were scanned to detect Texas Red (583 nm), FAM (494 nm), Cy5 (530 nm), and Yakima yellow (646 nm).