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. 2014 Apr 25;14(5):7580–7601. doi: 10.3390/s140507580

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© 2014 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 3. — Amplification products of RT-PCR consensus and serotype-specific primer pairs. The RT-PCR products were observed in ethidium bromide-stained agarose gels (2%). Input viral RNAs were extracted from each control dengue virus strain. Results for serotypes DEN-1, DEN-2, DEN-3, and DEN-4 are shown. (a) The 169-, 362-, 265-, and 426-bp amplicons for DEN-1, DEN-2, DEN-3, and DEN-4, respectively. (b) The 470-bp amplicons expected for all four dengue serotypes using the consensus primers. (c) The 362-bp amplicons for DEN-2 obtained from five samples.