Table 2.

Comparison of the ELISA performance in the present microwell array and 96-well plate.

		Microwell	96-Well Plate
Reagent consumption (μL)	Goat anti-h IgA solution	2	100
	Blocking buffer	2.5	200
	Antigen/sediment solution	2	100
	HRP-conjugated goat anti-h IgA solution	2	100
	Substrate solution	2.5	100
	Washing buffer	42	2400
Total reagents consumption (mL)		0.053	3
Assay time (min)		25	135
LOD (ng/mL)		0.05	5.19