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. 2014 Jun 19;5:200. doi: 10.3389/fphys.2014.00200

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Copyright © 2014 Kim, Kwon, Lee-Kwon, Choi, Sanada and Kwon.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TonEBP is sumoylated in response to hypertonicity. (A) HEK293 cells were transfected with various combinations of TonEBP, Ubc9, and Xpress-tagged SUMO1, SUMO2, or SUMO3, as indicated. After 20 h, the cells were cultured for an additional 4 h in isotonic (I) or hypertonic medium (H). Whole cell extracts (WCE) were prepared and immunoblotted (IB) for TonEBP. Positions of TonEBP and slow TonEBP bands are indicated. (B) Cells were transfected as above and WCE were immunoprecipitated using anti-Xpression antibody. Immunoprecipitates (top) and WCE (bottom) were immunoblotted for TonEBP. Positions of TonEBP and sumoylated TonEBP (SUMO-TonEBP) bands are indicated at right.