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Effects of sumoylation on DNA binding of TonEBP. Top: HEK293 cells were transfected with the constructs shown in Figure 4A. Cell lysates were prepared and immunoblotted for FLAG. Bottom: EMSA was performed on the cell lysates using 1 nM [32P]-labeled DNA containing TonE in the absence or presence of 100 nM unlabeled DNA containing TonE as indicated (Bottom). DNA bound TonEBP is indicated.
