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Proposed mechanism for the ISC Fe–S cluster assembly that is mediated by IscX as an iron supplier and as a regulator of cysteine desulfurase (IscS). The species S• is bound to the sulfur of a cysteine residue of either IscS or IscU as indicated, and S2–:Fe3+ is bound to a cysteine residue of IscU as Cys–S–Fe–S. See the text for details of each step.
