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. 2014 May 8;136(22):7933–7942. doi: 10.1021/ja501260h

Figure 9.

Figure 9

IscX and CyaY suppress iron–sulfur cluster assembly. Iron–sulfur cluster assembly on IscU was monitored by following absorbance at 456 nm. All reaction mixtures contained 0.5 μM IscS, 25 μM IscU, 5 mM DTT, 125 μM l-cysteine, and 125 μM ferrous ammonium sulfate: reaction mixture alone (black); reaction mixture with added 1.5 μM IscX (red); reaction mixture with added 25 μM IscX (blue); reaction mixture with added 25 μM CyaY (green). The entire UV–vis spectrum for each reaction is shown as a function of time in Figure S7.